The 28th IS is one of the oldest squadrons assigned to the 655th ISR Wing. It was activated Aug. 1, 1944, at Dalhart Army Air Field, Texas, as the 28th Photographic Laboratory, Bombardment Group, Very Heavy. The group was moved to Harvard AAF, Nebraska, a few weeks later and rotated through Hawaii and Tinian, Northern Mariana Islands, before ultimately ending up on Northwest Field, Guam, in support of combat operations in the Pacific Theater. The 28th PL earned a World War II campaign streamer for the Air Offensive, Japan. After World War II, the squadron was inactivated at Northwest Field on Feb. 22, 1946. While the 28th PL, Bombardment Group, Very Heavy was still inactive, the 28th Reconnaissance Technical Squadron was activated May 1, 1950, at Rapid City, South Dakota, (later Ellsworth Air Force Base) as a brand new squadron.

They served there until April 15, 1955, when they were inactivated. On March 19, 2014, while still inactive, the 28th RTS was re-designated as the 28th IS. They were reactivated April 17, 2014, at Hurlburt Field, Florida, and assigned to the 655th ISR Group. The 28th PL was reconstituted and consolidated with the 28th IS Sept. 18, 2014, which made these two squadrons one with a shared history. The newly reborn 28th IS was tasked to deliver specialized collection, analysis and dissemination directly to their active-duty partners: Air Force Special Operations Command and 25th IS, which is a subordinate unit of the 363rd ISR Wing at Joint Base Langley-Eustis, Virginia.

The 38th IS was constituted and activated Oct. 26, 2013, at Beale Air Force Base, California, and was assigned to the 655th Intelligence, Surveillance, and Reconnaissance Group. It was later reassigned to the 755th ISR Group. The 38th IS maintains an active-duty partnership with the 13th IS, which is a subordinate organization of the 480th ISR Wing at Joint Base Langley-Eustis, Virginia.

The 42nd IS was originally activated Sept. 2, 1945, at Smokey Hill Army Air Field, Kansas, as the 42nd Photo Lab, Bomb Group Very Heavy. They were part of the 456th Bombardment Group, Very Heavy, which was already a veteran of multiple campaigns overseas, including raids on the oil refineries at Ploiesti, Romania, in May 1944. The 456th BG was relocated to Smokey Hill AAF, Tennessee, to convert to the B-29, but the war ended before the conversion was completed and the group was inactivated. The 42nd PL was inactivated along with the 456th BG on Oct. 17, 1945. The 42nd PL was re-designated as the 42nd IS on Aug. 19, 2013, and activated in the Reserve on Sept. 15, 2013, at Joint Base Langley-Eustis, Virginia, under the 655th Intelligence, Surveillance, and Reconnaissance Group. It was later reassigned to the 755th ISR Group. The 42nd IS maintains an active-duty partnership with the 36th IS, a subordinate unit of the 636th ISR Wing at JBLE.

The 50th IS was initially activated Nov. 1, 1974, at Ramstein Air Base, Germany, as the 7450th IS under the 86th Tactical Fighter Wing. In December 1979, they were realigned underneath U.S. Air Forces in Europe. On Sept. 1, 1985, they were realigned under the 7455th Tactical Intelligence Wing where they remained until being inactivated July 1, 1994. They were re-designated as the 50th IS on Sept. 21, 2007, and then reactivated Oct. 1, 2007, at Beale Air Force Base, California. They were aligned first to the 610th Regional Support Group until July 1, 2009, when they were realigned under the 940th Operations Group. They were realigned to the 655th Intelligence, Surveillance, and Reconnaissance Group on Sept. 15, 2013, and later moved to the 755th ISR Group.

The 63rd IS was originally activated Sept.19, 1944, at Dalhart Army Air Field, Texas, as the 33rd Photo Lab, Bomb Group Heavy and assigned to the 382nd Bombardment Group. The 382nd BG was reactivated in September 1944 as a B-29 unit. The unit deployed to the island of Ie Shima, Japan, from September to December 1945 and was awarded the World War II Asiatic-Pacific Theater service streamer for their service. In December 1945, the squadron was redeployed and ultimately inactivated in January 1946 at Camp Anza, California. The squadron was redesignated as the 63rd IS on Aug. 19, 2013, and reactivated Sept. 15, 2013, at Joint Base Langley-Eustis, Virginia. The 63rd IS maintains an active-duty partnership with the 30th IS, a subordinate organization to the 480th Intelligence, Surveillance, and Reconnaissance Wing at JBLE.

The 718th IS was activated May 1, 2011, at Joint Base Langley-Eustis, Virginia, as a subordinate organization of the 940th Operations Group at Beale Air Force Base, California. It was one of two squadrons whose activation date precedes the 655th Intelligence, Surveillance, and Reconnaissance Group activation because it was already active when the group was activated in 2013. The 718th IS maintains a classic association partnership with the 45th IS and the 10th IS, both of which are subordinate to the 480th ISR Group at JBLE.

The 820th IS, formerly Detachment 3, Offutt Air Force Base, Nebraska, was activated Nov. 4, 2017, at Offutt AFB and assigned to the 655 Intelligence, Surveillance, and Reconnaissance Group as a geographically separated unit. It was later reassigned to the 755th ISR Group. It is a targeting intelligence squadron tasked to provide target analysis and aim point development to its active-duty partner, the 20th IS, a GSU of the 363rd ISR Wing at Joint Base Langley-Eustis, Virginia.